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KMID : 0365819730130010215
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Journal of Pusan Medical College
1973 Volume.13 No. 1 p.215 ~ p.221
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The Effect of Organic Phosphorus and Chlorinated Hydrocarbon Compounds on 3β-Hydroxysteroid Dehydrogenase Activity in Adreanls of Rat
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Abstract
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Recently, dipterex, DDVP and EPN have attracted wide interests as insecticides. The toxicities and mechanisms of action of these agents have not as yet been explained thoroughly.
So far as I kno , the work reported here is the first attempt specifically designed to study the effect of organic phosphorus n ad chlorinated hydrocarbon compounds on 3(3-hydroxysteroid dehydrogenase activity in, adrenals of rat.
Rats were used as experimental animals throughout this study. Sixty rats were divided into 12 groups;
Group 1: control, Group 2: EPN injected. Gronp 3: dipterex injected, Group 4: DDVP injected, Group 5: DDT injected, Group 6: BHC injected, Group 7: EPN plus DDT injected, Group 8: EPN plus BHC injected, Group 9: dipterex plus DDT injected, Group 10: dipterex plus BHC injected, Group 11: DDVP plus DDT injected, Group 12: DDVP plus BHC injected.
The activity was determined at 37°C by monitoring formation of DPNH at 340 mμ in a Beckman DU spectrophotometer. Each cuvette contained 0.75 ml. of homogenate, 50 mM sodium phosphate buffer(pH 7.2), 40 mM nicotinamide, 5 mM disodium EDTA, 7 mM (3-mercaptoethanol,1 mM KCN, 0.3 mM DPN+ and the designated amounts of substrate in 0. 1 ml. of propylene glycol with a total volume of 3.0ml.. The results of this investigation were as follows:
1. 3j3-Hydroxysteroid dehydrogenase activities of adrenals of rat determined with 6-estradiol, allopregnanolone, and 17x- hydroxypregnenolone as its substrate were 550, 224, and 2O5μ mole of product/ min. /g of wet tissue, respectively.
2. 3(3-Hydroxysteroid dehydrogenate activity was inhibited by EPN, dipterex, DDVP and DDT when determined with (3-estradiol as a substrate.
3. 3(3-Hydroxysteroid dehydrogenase activity was inhibited by EPN plus DDT, dipterex plus DDT, and DDVP plus BHC when determined with (3-estradiol as a substrate.
4. W-Hydroxystero d dehydrogenas^ activity was activated by dipterex plus BHC and DDVP plus BHC, but it was inhibited by dipterex plus DDT, when determined with allopregnanolone as a substrate.
5. 3/3-Hydroxysteroid dehydrogenase activity was inhibited by DDVP plus DDT and DDVP plus BHC, when determined with 17a-hydroxypregnenolone as a substrate.
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KEYWORD
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